Principal Investigator(s): Katherine East, Inga Zasada, R. Paul Schreiner, and Michelle Moyer

Project Duration: 2015-2017


Meloidogyne hapla is the most prevalent plant-parasitic nematode in Washington state wine grape vineyards. Understanding the developmental dynamics of M. hapla can improve the timing of diagnostic sampling and nematicide application. Three Vitis vinifera vineyards in Washington were sampled March 2015 to March 2017 to determine the developmental dynamics of M. hapla by measuring second-stage juveniles (J2) in soil, eggs and adult females in roots, and fine root tips. A model of M. hapla J2 development based on soul growing degree days using a base temperature (Tb) of 0*C (GDD soil) and a start date of 1 March was developed. This model was validated at two additional vineyards in Washington and was robust with R2 values >0.74. M. hapla has one generation per year and overwinters primarily as the J2 infective stage. Juvenile populations declined after 1 March, reaching their lowest density in early July and reaching a maximum density over the winter. M. hapla egg and root tip densities reached a maximum in early August. The number of females per root tip did not vary throughout the year. A single generation with defined peaks in J2 population densities will allow for specific timing of nematicide interventions.

Pest & Disease // Viticulture //